Motility Assays for Motor Proteins


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Diatrack Vallotton and Olivier, ; Vallotton et al.

Thus, ActiPHV improves the result compared to existing approaches. From our experiments it turned out that ActiPHV yields a higher accuracy compared to previous methods, which enhances the sensitivity and accuracy of the assay. We used manual tracking data and automatically determined tracking data.

Introduction

Since manual tracking is very time-consuming and therefore not appropriate for the analysis of IVMA images in daily labwork, accurate automatic tracking methods are essential. Different methods for filament tracking in IVMA images have been introduced. In our work, we used Diatrack Vallotton and Olivier, ; Vallotton et al. This tracking method yields good results when detection parameters are adjusted manually for each image sequence.

Even though there are partly tracking errors data not shown , they hardly influence the result of ActiPHV for the maximum velocity, which shows that our method is robust. Thus, our method advances the analysis of IVMA images and enables to better exploit the power of the assay. KR supervised the development of the method, while L-MS and RF supported the work from the laboratory point of view by regular review and discussion of intermediate results. NB drafted the code article, which was critically revised by all authors.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Aksel, T. Ensemble force changes that result from human cardiac myosin mutations and a small-molecule effector. Elangovan, R. An integrated in vitro and in situ study of kinetics of myosin ii from frog skeletal muscle.

Gordon, A. Calcium regulation of skeletal muscle thin filament motility in vitro. In vitro motility speed of slow myosin extracted from single soleus fibres from young and old rats. Kalman, R. A new approach to linear filtering and prediction problems.

Basic Eng. Kron, S. Fluorescent actin filaments move on myosin fixed to a glass surface. Luethi, D. R package version 0. Meijering, E. Methods for cell and particle tracking.

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Spiral Defects in Motility Assays: A Measure of Motor Protein Force

Methods Enzymol. Rastogi, K. Maximum limit to the number of myosin II motors participating in processive sliding of actin. Scheid, L.

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Extraction protocols for individual zebrafish's ventricle myosin and skeletal muscle actin for in vitro motility assays. Essential light chain S phosphorylation is required for cardiac adaptation under physical stress. Uttenweiler, D.

Cytoskel MTs Motor proteins

Motion determination in actin filament fluorescence images with a spatio-temporal orientation analysis method. Vallotton, P. Tri-track: free software for large-scale particle tracking. Diatrack particle tracking software: Review of applications and performance evaluation.


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Traffic 18, — Yengo, C. Temperature dependent measurements reveal similarities between muscle and non-muscle myosin motility. Muscle Res. Keywords: myosin, actin, in vitro motility assay, filament, velocity, signal reconstruction, code:R. The use, distribution or reproduction in other forums is permitted, provided the original author s and the copyright owner s are credited and that the original publication in this journal is cited, in accordance with accepted academic practice.

No use, distribution or reproduction is permitted which does not comply with these terms. View Preview. Learn more Check out. Abstract Light microscopic motility assays provide critical information about the mechanism of action of motor proteins. Citing Literature.

Motility Assays for Microtubule Motor Proteins

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G3b motility assay - Biophysics and Molecular Materials - LMU Munich

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    Motility Assays for Motor Proteins Motility Assays for Motor Proteins
    Motility Assays for Motor Proteins Motility Assays for Motor Proteins
    Motility Assays for Motor Proteins Motility Assays for Motor Proteins
    Motility Assays for Motor Proteins Motility Assays for Motor Proteins
    Motility Assays for Motor Proteins Motility Assays for Motor Proteins
    Motility Assays for Motor Proteins Motility Assays for Motor Proteins

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